ラボプロトコール集
Contents
| I. Biochemical Protocol | |||
|
I-１
|
Quantitation of Proteins (1) BCA assay (2) Bradford |
 |
|
|
I-2
|
Preparation of Cell Lysate |
|
|
|
I-3
|
SDS-PAGE |
|
|
|
|
I-4
|
Immunoblot (Western Blotting) |
|
|
|
I-5
|
Expression and Purification
of GST-fusion Proteins
|
|
 |
|||
| II. Proteome Protocol | |||
|
|
II-1
|
Two Dimensional Gel Electrophoresis (2-DE) |
|
|
II-2
|
SYPRO Ruby Protein and ProQ Diamond
Phosphorprotein Gel Staining |
|
|
|
|
II-3
|
In Gel Tryptic Digestion
|
|
|
II-4
|
Identification of Targeted-proteins
Using AXIMA-QIT/TOF (Shimadzu Biothch, Kyoto, Japan). |
|
|
|
|
|||
|
III. Cell
Biological Protocol
|
|||
|
III-1
|
DNA Transfection Mediated by Lipofection |
|
|
|
|
III-2
|
Immunocytochemistry |
|
|
III-3
|
Immunocytochemistry (Uptake of Alexa-conjugated Tf) |
|
|
|
|
III-4
|
Primary Cultures of Neurons and Astrocytes in Brain |
|
|
|
III-5
|
Primary Culture of Embryonic Mouse
DRG Neurons
|
|
|
III-6
|
Culture of Rat Oligodendrocytes | |
|
|
III-7
|
Culture of Mouse Schwann Cells | |
|
|
|
|||
| IV. Behavioral Experiments in Rodents | |||
|
IV-1-1
|
Irwin's multi behavioral tests |
|
|
| IV-1-2 | ANESTHESIA | |
|
| IV-2-1 |
Convulsive Seizures induced by Pentylentetrazole (PTZ) single injection (Generalized seizure model) |
|
|
|
IV-2-2
|
Pentylentetrazole (PTZ) induced Kindling Model |
|
|
|
IV-3
|
Kainate-induced Status Epilepticus (Temporal Lobe Epilepsy model) | |
|
|
IV-4
|
Pilocarpine-induced Status Epilepticus (Temporal Lobe Epilepsy model) | |
|
|
|
|
||
Copyright(C)2006 Tokushima Bunri University.All
Rights Reserved